电离辐射通过转化生长因子-β-介导的上皮-上皮转换来促进癌细胞的侵袭迁移

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

摘要 ：论述紫外支线到底可通过裂解激素-β（TGF-β）-介导的黏膜-上皮细胞转换 (EMT)来促使免疫细胞会的侵扰搬迁。适用总量2Gy(60)Coγ支线紫外线来源于生物循环系统的6种免疫细胞会，记录与EMT相关的变化，这还包括分别借助于显微技术，酶质马尔季尼夫卡方法，免疫荧光技术，划痕试验和Transwell小室试验来判读并验证细胞会组织形态，EMT标明，侵扰搬迁能够等。采用酶同德免疫吸附法验证这些免疫细胞会之前TGF-β酶素质，借助于特别抑制剂SB431542来指标TGF-β波形通道在紫外支线EMT之前的作用。经过总量为2Gy紫外线的免疫细胞会之前假定间叶细胞会的理解，与假紫外线组相比其黏膜标明减少，间叶细胞会标明增加，同时其侵扰移出能够增强，TGF-β酶素质也进一步提高。再进一步辨认出由A549紫外支线抑制的EMT可通过对TGF-β波形抑制发生逆转。这些结果表明TGF-β介导的EMT在紫外支线抑制增强免疫细胞会侵扰移出能够之前起着关键作用。

出版人： lizexiu